Alkaloids from Anadenanthera colubrina seeds were extracted using Ott's [1]
method, except that methanol was used instead of ethanol, and methylene
chloride (= dichloromethane) replaced the chloroform. Tartaric acid is
easily obtained OTC from a pharmacy, it is used as a flavouring in soft
drinks and for home brewing. Hydrochloric acid and ammonium hydroxide are
both available from hardware stores. 

Ott describes a 'foamy yellowish oil', I can confirm this is exactly
as the product appears. No attempt at recrystalization has yet been made
as the seed suppliers lab has found that in addition to the primary
alkaloid bufotenine, both DMT and 5-MeO-DMT are also present in relatively
large quantities; this is surprising because Ott reports these two alkaloids
occur only in trace quantities [Note by tacethno.com: not that surprising];
the potential synergy between all three compounds is more interesting to me
than just the pharmacology of bufotenine which Ott was experimenting with. 

The total yeild from these seeds was in accodnace with Ott's findings,
between 3-4%; this  figure is very good news, the active dose for inhaled
vapour being < 10 mg, so a little will go a long way :)

Future research will concentrate on the use of A. colubrina extract
as an addmixture in combination with Mimosa hostilis and Peganum harmala
(MAOI) extracts. [Note by tacethno.com: do not attempt this at home,
this combination is untested and potentially dangerous!]

Highest,
Al


[1] Ott, J; Pharmanopo-Psychonautics; Journal of
Psychoactive Drugs, Vol. 33 (3), July-September 2001.
http://www.tacethno.com/info/bufotenine/pharmanopo.html